ReaMetrix Custom Services has extensive expertise in the development of cell lines that permanently express proteins of interest at high, homogeneous levels. We provide a complete development solution, including:

Typical Cell Line Development Synopsis

Isolation or acquisition of gene of interest

Cloning of gene into suitable expression vector - we work with our customers to determine the vector that is most desirable for the ultimate application

Determine the sensitivity of the parent cell line to specific concentrations of selection agent

Optimization of transfection conditions for the specific host cell line

Linearization of vector and generation of stable clones

Screening of stable clones for optimal expression, in accordance with desired specifications (cell surface expression, Southern Blot, Western Blot, Northern Blot, etc.)

Verification of mycoplasma-free status of stable clones

Typical Cell Line Development Timeline:

Creating a stable cell line can take a relatively short amount of time (1-2 months) if the expression vector has already been developed and the host cell line is amenable to tranfection. Other projects, where multiple genes are inserted into the host cell genome sequentially, can take significantly longer. At ReaMetrix, we evaluate each cell line project individually, and create a development program designed to yield a high-quality cell line meeting the desired specifications.

Pricing and Customer Service:

We strive to maintain the highest level of quality, reliability, and customer services at very competitive prices. Our dedicated customer service representatives will assist you at each step to provide technical assistance, timeline development, pricing information, and support you through the entire development process.

For an analysis of your specific cell line project and an individual quote, please contact us at custom@reametrix.com. Please provide as many details as you can about the project and cell lines you want to work with. We will contact you within 24 hours to provide an initial estimate or to request additional information that we may need to provide an accurate quotation.

Examples of GPCR expressing cell lines developed at ReaMetrix

Stable CHOKI cells expressing IL8RB stained with fluorescently labeled chemokine IL8	Stable CHOKI cells expressing  CXCR4 stained with fluorescently labeled  SDFI alpha
Control untransfected cells showed almost negligible staining with labeled chemokine